antimouse leptin r antibody  (R&D Systems)

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Estradiol affects extracellular leptin:adiponectin ratio in human breast tissue in vivo.

doi: 10.1210/jc.2014-1129

Figure Lengend Snippet: Figure 1. Leptin increased and adiponectin decreased in normal human breast tissue exposed to estrogen. Microdialysis sampling was used to collect extracellular adipokines in normal human breast tissue and subcutaneous (sc) abdominal fat from both pre- and postmenopausal women. A. Plasma estradiol levels positively correlated with extracellular leptin level (r 0.37; P .05; n 39) and negatively correlated with the local extracellular adiponectin level (r-0.34; P .05; n 39) and positively correlated with the leptin:adiponectin ratio (r 0.38; P .05; n 39) in normal human breast tissue. B. Plasma estradiol did not correlate with local extracellular adipokine levels in sc abdominal fat or with plasma adipokine levels.

Article Snippet: Immunohistochemistry Formalin-fixed, paraffin-embedded normal breast tissue biopsies and mouse tumors were cut into 4- m sections, deparaffinized, and subjected to high-temperature antigen retrieval prior to incubating with the following primary antibodies: rabbit antihuman leptin (1 g/ml; GenWay Biotech, San Diego, CA, USA), goat antihuman LepR (20 g/ml; AbD Serotec, Düsseldorf, Germany), goat antimouse leptin (10 g/ml; R&D Systems), goat antimouse LepR (10 g/ml; R&D Systems), rabbit antihuman adiponectin (1 g/ml; Abcam, Cambridge, UK), rabbit antihuman AdipoR1 (5 g/ml; Bioss, London, UK), and rabbit antihuman AdipoR2 (5 g/ml; Bioss).

Techniques: Sampling, Clinical Proteomics

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Estradiol affects extracellular leptin:adiponectin ratio in human breast tissue in vivo.

doi: 10.1210/jc.2014-1129

Figure Lengend Snippet: Figure 2. Leptin increased and adiponectin decreased in normal breast tissue exposed to estrogen. A. Healthy premenopausal women (n 11) underwent microdialysis sampling of normal breast tissue and subcutaneous (sc) abdominal fat tissue in the follicular (Foll) and luteal (Lut) phases of a single menstrual cycle. *P .05, **P .01, ***P .001. B. To confirm the in vivo findings, whole normal human breast tissue biopsies were cultured with or without 10-9 M estradiol (E2) or a combination of 10-9 M estradiol (E2) and 10-8 M progesterone (E2 P4) for 7 days, and adipokine levels in the culture medium were analyzed. **P .01 compared to controls (each group, n 9). C. Normal breast tissue biopsies were cultured ex vivo with or without 10-9

Article Snippet: Immunohistochemistry Formalin-fixed, paraffin-embedded normal breast tissue biopsies and mouse tumors were cut into 4- m sections, deparaffinized, and subjected to high-temperature antigen retrieval prior to incubating with the following primary antibodies: rabbit antihuman leptin (1 g/ml; GenWay Biotech, San Diego, CA, USA), goat antihuman LepR (20 g/ml; AbD Serotec, Düsseldorf, Germany), goat antimouse leptin (10 g/ml; R&D Systems), goat antimouse LepR (10 g/ml; R&D Systems), rabbit antihuman adiponectin (1 g/ml; Abcam, Cambridge, UK), rabbit antihuman AdipoR1 (5 g/ml; Bioss, London, UK), and rabbit antihuman AdipoR2 (5 g/ml; Bioss).

Techniques: Sampling, In Vivo, Cell Culture, Ex Vivo

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Estradiol affects extracellular leptin:adiponectin ratio in human breast tissue in vivo.

doi: 10.1210/jc.2014-1129

Figure Lengend Snippet: Figure 3. Tamoxifen significantly decreased leptin and increased adiponectin in normal human breast tissue. A. Postmenopausal women underwent microdialysis sampling of breast tissue (n 18) and subcutaneous (sc) abdominal fat (n 16) before tamoxifen treatment and 6 weeks after treatment. *P .05, **P .01. B. To confirm the in vivo findings, whole normal human breast tissue biopsies were cultured with or without 10-6 M tamoxifen (Tam) for 7 days, and adipokine levels in the culture medium were determined. *P .05 (n 9), ****P .0001 (n 12) compared to controls. C. Normal breast tissue biopsies were cultured ex vivo with or without 10-6 M tamoxifen (Tam). Levels of adipokines and their corresponding receptors (leptin receptor [LepR], adiponectin receptor 1 [AdipoR1], and adiponectin receptor 2 [AdipoR2]) were determined by immunohistochemistry analysis. No differences were detected between treatment groups. Representative tissue sections from each treatment group are shown (scale bars, 20 m).

Article Snippet: Immunohistochemistry Formalin-fixed, paraffin-embedded normal breast tissue biopsies and mouse tumors were cut into 4- m sections, deparaffinized, and subjected to high-temperature antigen retrieval prior to incubating with the following primary antibodies: rabbit antihuman leptin (1 g/ml; GenWay Biotech, San Diego, CA, USA), goat antihuman LepR (20 g/ml; AbD Serotec, Düsseldorf, Germany), goat antimouse leptin (10 g/ml; R&D Systems), goat antimouse LepR (10 g/ml; R&D Systems), rabbit antihuman adiponectin (1 g/ml; Abcam, Cambridge, UK), rabbit antihuman AdipoR1 (5 g/ml; Bioss, London, UK), and rabbit antihuman AdipoR2 (5 g/ml; Bioss).

Techniques: Sampling, In Vivo, Cell Culture, Ex Vivo, Immunohistochemistry

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Estradiol affects extracellular leptin:adiponectin ratio in human breast tissue in vivo.

doi: 10.1210/jc.2014-1129

Figure Lengend Snippet: Figure 4. Increased extracellular leptin and decreased adiponectin in human breast cancer in vivo. A. Eleven postmenopausal breast cancer patients underwent microdialysis sampling of the tumor and normal adjacent breast tissue before surgery. *P .05, **P .01. B. FVB/N mice were oophorectomized and treated with or without estradiol (physiologic level), and MMTV- PyMT mammary cancer cells were injected into the dorsal mammary fat pad. At similar tumor sizes, both groups of mice underwent microdialysis sampling of adipokines in the tumor. *P .05, **P .01 (each group, n 7). C. FVB/N mice were treated as described in Figure 4B. Levels of adipokines and their corresponding receptors (leptin receptor [LepR], adiponectin receptor 1 [AdipoR1], and adiponectin receptor 2 [AdipoR2]) in the MMTV-PyMT tumors were determined by immunohistochemistry analysis. Increased LepR staining was observed in estrogen-exposed tumors. Representative tissue sections from each treatment group are shown (scale bars, 20 m).

Article Snippet: Immunohistochemistry Formalin-fixed, paraffin-embedded normal breast tissue biopsies and mouse tumors were cut into 4- m sections, deparaffinized, and subjected to high-temperature antigen retrieval prior to incubating with the following primary antibodies: rabbit antihuman leptin (1 g/ml; GenWay Biotech, San Diego, CA, USA), goat antihuman LepR (20 g/ml; AbD Serotec, Düsseldorf, Germany), goat antimouse leptin (10 g/ml; R&D Systems), goat antimouse LepR (10 g/ml; R&D Systems), rabbit antihuman adiponectin (1 g/ml; Abcam, Cambridge, UK), rabbit antihuman AdipoR1 (5 g/ml; Bioss, London, UK), and rabbit antihuman AdipoR2 (5 g/ml; Bioss).

Techniques: In Vivo, Sampling, Injection, Immunohistochemistry, Staining

Journal: The Journal of nutritional biochemistry

Article Title: Changes in liver PPARalpha mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice.

doi: 10.1016/j.jnutbio.2006.03.003

Figure Lengend Snippet: Fig. 1. Serum leptin concentration of Wistar rats and C57BL/6J mice fed diets containing 5 g/100 g (5% SFO), 20 g/100 g (20% SFO) or 30 g/100 g (30% SFO) SFO for 13 weeks (Wistar rats) or 22 weeks (C57BL/6J mice). Values are meanFS.E.M.; n =9–10 (rats) or 10–13 (mice). Data were analyzed by one-way ANOVA and Duncan’s multiple range test for the significance of difference among the three diet groups of each species. Values not sharing a common letter among the three diet groups of the same species are significantly different ( P b.05).

Article Snippet: Serum leptin was measured by enzyme-linked immunosorbent assay using a goat antimouse leptin antibody (R&D Systems, Minneapolis, MN, USA) with high affinity for rat and mouse.

Techniques: Concentration Assay